Test Preparation

1. Allow all kit components to equilibrate to room temperature (15-30°C) prior to testing for 30 minutes,if previously stored in a cool place.

2. Do not eat, drink, smoke, brush teeth, or chew gum for 30 minutes before collecting saliva.

Specimen Collection & Extraction

1. Spit the expectorated saliva into the collection funnel gently as much as possible close to 1mL.

2. Unscrew the collection funnel gently to allow the saliva flow into the collection tube completely.

3. Screw the nozzle onto the collection tube.

4. Turn upside down the collection tube slightly for 5 seconds, so that the diluent can mix with the saliva sample evenly.

5. Leave the collection tube stand at room temperature for 5 minutes.

6. Remove the nozzle cap.

Reaction with Test Cartridge

7.Remove a test cartridge from the sealed pouch by tearing at the notch and place it on a level surface.Drip vertically 2~3 drops of liquid at

2~3 second intervals into the specimen well (S) on the test cartridge by squeezing the tube. Do not handle or move the test cartridge until

the test is complete and ready for reading.

8. Start timer. Read result within 15 ~20 minutes of adding the liquid . The test result is invalid after 20 minutes.