>2019-nCoV Nucleic Acid Detection Kit<br>(Q-PCR Method)
2019-nCoV Nucleic Acid Detection Kit
(Q-PCR Method)
  • Model : LS-C-T-001
  • Intro : The Kit is a real-time multiplex RT-PCR test intended for the qualitative detection of 2019-nCoV viral RNA. It is suitable for upper respiratory specimens, such as anterior nasal swab, nasopharyngeal swab, oropharyngeal swab, and saliva specimens etc. In a single RT-PCR reaction, it is able to detect ORF1ab and N RNA sequences, and in addition, human RNase P as an internal control.

    For in vitro diagnostic use only. For professional use only.


















ItemContentsQuantity 96 Test/kit
RT-PCR Mix，lyophilized microspherePrimers, probes, reaction buffer、Mg2+、dNTPs、M-MLV Reverse Transcriptase、Taq DNA Polymerase8-tube strip × 12
Positive Control （PC）， lyophilized microspherePseudovirusTube ×1

【Test Procedure】


1. Nucleic acid extraction


Commercial viral nucleic acids kits have been found to be able to extract high quality RNA when manufacturer's 


recommended procedures are followed.


2. Positive control reconstitution


Add 400 μL nuclease-free water  to the Positive Control tube, and mix well before use.


3. RT-PCR Reaction Setup


a. Take  N+2 tubes of RT-PCR Mix 8-tube strip, N refer to the number of samples to be tested.A single-reaction amplification system is  prepared as follows:


































Nuclease-free waterSpecimen RNAPositive control 
Specimen tubes13 μL5 μL(specimen processed by nucleic acid release reagent)-
-18 μL (specimen RNA extracted by nucleic acid extraction kit )-
Positive control tube--18 μL
Negative control tube18 μL--

b. Close caps and spin briefly. 


c. Place the reaction tubes into the specimen rack of the real-time PCR instrument.


4. RT-PCR protocols:


Recommended Settings









































StepTemperature（℃）TimeCycle
ⅠReverse Transcription5010 min1
ⅡPre-denaturation952 min1
ⅢDenaturation955 s45
Annealing / extension6020 s
*Fluorescence signal is detected at 60 °C in step Ⅲ and the fluorescence channels are: FAM, VIC, and Cy5.

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